Poster Authors and Abstracts CSU Annual Biotechnology Symposium. CSU Biotechnology Symposium. Posters with Author Listings and Abstracts. Use Find function or Ctrl F to search. Poster 1 Campus CSU Northridge. Poster Category AgricultureBiofuelsEnvironment. Keywords Arabidopsis thaliana, Genetic engineering, Weather pollution. Project Title Overexpression of an aminocyclopropane 1 carboxylic acid synthase gene in Arabidopsis thaliana and its effect on removing environmental pollutants. Author List Ramadoss, Niveditha Graduate, Biology, California State University, Northridge, Presenting Author. Gupta, Dinesh Washington University, St. Louis. Guenther, Alex University of California, Irvine. Basu, Chhandak Biology, California State University, Northridge. Abstract Rise in air pollution has led to increasing cancer, several respiratory and cardiac illnesses in humans. Advantages And Disadvantages Of Evolutionary Model In Software Engineering' title='Advantages And Disadvantages Of Evolutionary Model In Software Engineering' />World Health Organization estimates that one in eight person die from air pollution. The main objective of our study is to analyze the feasibility of genetically engineered plants to remove polluting volatile compounds from air. To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS Aminocyclopropane 1 carboxylic acid synthase gene. The spiral model is a riskdriven process model generator for software projects. Based on the unique risk patterns of a given project, the spiral model guides a team. Advantages And Disadvantages Of Evolutionary Model In Software Engineering' title='Advantages And Disadvantages Of Evolutionary Model In Software Engineering' />A useful revision guide looking at the benefits and disadvantages of structured interviews and postal questionnaires, for GCSE sociology. CSU Biotechnology Symposium Posters with Author Listings and Abstracts. Use Find function or Ctrl F to search. Poster 1 Campus CSU Northridge. SDLC Quick Guide Learn Software Development Life Cycle starting with SDLC overview and covering various models like waterfall model, Iterative Model, Spiral Model. Software prototyping is the activity of creating prototypes of software applications, i. NOCTI, and Nocti Business Solutions NBS, an NCCRS member since May 2012, is a leading provider of highquality technical competency assessment products and services. Using quantitative real time PCR q. PCR, we previously confirmed that the ACS gene was upregulated in poplar Populus trichocarpa plants when exposed to environmental pollutants including ozone and methyl vinyl ketone. Therefore, we hypothesize that ACS gene might play an important role in poplar plants in mitigating stresses resulting from pollutant exposure. The homolog of poplar ACC synthase gene was PCR amplified from Arabidopsis thaliana and cloned into p. BINmgfp. 5 er vector, featuring a constitutive cauliflower mosaic virus promoter removing the native jellyfish green fluorescent GFP gene. The recombinant plasmid containing the ACS gene was electroporated into the GV1. Agrobacterium tumefaciens. Arabidopsis plants were genetically engineered with ACS gene by dipping the T0 transgenic zero or wild type flowering plants into GV1. Agrobacterium containing the recombinant binary vector. The transformed plants were selected through kanamycin resistance and grown till T3 third transgenic generation. The ACS gene expression of these plants will be confirmed through q. PCR. The pollutant absorbing capacity will be tested by placing the transformed T3 plants in an environmental gas chamber where they will be exposed to various levels of pollutants. The ability of the transformed plants to absorb pollutants will be evaluated by performing gas chromatography mass spectrometry analyses to measure pollutants in the inlet and outlet airflow in order to quantify the pollutant deposition rate. Our use of a model plant Arabidopsis is proof of concept. Our future goal will be to transform tree species and evaluate their potentials to remove pollutants. These studies will open doors for use of transgenic plants to clean up environments. Poster 2 Campus Sonoma State University. Poster Category AgricultureBiofuelsEnvironment. Keywords Biosensors, Wireless Biosensor Network, Soil. Project Title A Comparative Study of RF Wave Attenuation in Soil and Sand. Author List Palmerin, Abraham Undergraduate, Engineering Science, Sonoma State University, Presenting Author. Farahmand, Farid Engineering Science, Sonoma State University. Fong, Erin Lawrence Berkeley National Laboratory, Engineering Division. Abstract Soil is the most spatially complex stratum of a terrestrial ecosystem. Therefore, soil monitoring and understanding changes in soil ecosystem are considered to be important aspects of any environmental monitoring. The recent emergence of Wireless Biosensor Networks WBNs promises addressing many ecological questions, particularly related to soil, though providing uninterrupted real time data from various biosensors using RF signals. In this work we focused on understanding the impact of soil composition e. RF waves travel underground. This study is crucial in order to define the appropriate RF band, power level, and RF antenna size for extended underground monitoring. Free Pdf Printer Software Download. Our research focused on 5. GHz ISM bands. The first part of our experiment was conducted in a 1. We measured the RF signal path loss through the sand for the above frequencies at different horizontal distances between the transmitter and receiver. We also measured RF signal strength as we changed the vertical distances between the two antennas. In the second part of our experiment, we used regular gardening soil mostly composed of organic matter, perlite and vermiculite. Vermiculite keeps the soil hydrated and is part of the clay minerals group. We made similar measurements. In both cases, we maintained the temperature at 2. Celsius. We normalized the obtained path loss measurements as a function of distance for all frequencies to remove any offset difference due to factors such as antennas. Our results indicate that at 0. GHz the received signal power level decays with distance by about 1. Bcm when traveling in soil. We also found that at higher frequencies, the RF signal experiences 4 6 d. B more attenuation in soil than air. The exact value depends on the frequency and antenna polarization type. On the other hand, the path loss in sand is about 0. B more than air. It is therefore, preferred to use lower band frequencies for underground biosensors. However, the trade off will be antenna size and its design complexity. Our results conclude that it is critical to carefully manage signal power and understand antenna design when wireless biosensors are placed in an area with high quantity of clay content. This study was carried out in collaboration with Lawrence Berkeley National Laboratory, Division of Earth Sciences. Poster 3 Campus CSU Long Beach. Poster Category AgricultureBiofuelsEnvironment. Keywords Arabidopsis, Senescence, Epigenetics. Project Title A TIR NBS LRR Class Disease Resistance Protein Mays be a Positive Regulator of Developmental Senescence in Arabidopsis thaliana. Author List Mendoza, Victor Undergraduate, Chemistry Biochemistry, California State University, Long Beach, Presenting Author. Brusslan, Judy Biological Sciences, California State University, Long Beach. Abstract Leaf senescence is the final stage in leaf development in which the leaf reallocates its nutrients to growing parts of the plant. This study aims to determine if the At. TIR NBS LRR class disease resistance protein DRP, is involved in leaf senescence in Arabidopsis thaliana. This gene is up regulated in leaf senescence and the increase in expression occurs in parallel to increases in the H3. K4me. 3 mark. Two distinct transfer DNA T DNA insertion lines that disrupt At. DNA sequencing. Loss of gene expression was confirmed by amplification of c. DNA using insertion specific primers. Chlorophyll and total protein levels were used as physical markers for age. Differences between wild type and mutant leaves were quantified and compared using two tailed students t test. The transcript levels of robust senescence up regulated genes WRKY7. NIT2, were also compared. Our results revealed a significant increase of protein levels in the k. A trend of increased chlorophyll levels in k. Quantitative real time PCR data revealed no trends of increased senescence associated gene transcript levels.